The Effects of Dietary Supplementation of Lactococcus lactis Strain Plasma on Skin Microbiome and Skin Conditions in Healthy Subjects-A Randomized, Double-Blind, Placebo-Controlled Trial.

Ryohei Tsuji,Kamiyu Yazawa,Yuumi Nakamura,Takeshi Kokubo,Osamu Kanauchi

doi:10.3390/microorganisms9030563

Ryohei Tsuji, Kamiyu Yazawa + Show 3 more

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https://doi.org/10.3390/microorganisms9030563

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Journal: Microorganisms	Publication Date: Mar 9, 2021
Citations: 4	License type: CC BY 4.0

Affiliation: Kirin (Japan), Chiba University, Osaka University

Abstract

(1) Background: Lactococcus lactis strain Plasma (LC-Plasma) is a unique strain which directly activates plasmacytoid dendritic cells, resulting in the prevention against broad spectrum of viral infection. Additionally, we found that LC-Plasma intake stimulated skin immunity and prevents Staphylococcus aureus epicutaneous infection. The aim of this study was to investigate the effect of LC-Plasma dietary supplementation on skin microbiome, gene expression in the skin, and skin conditions in healthy subjects. (2) Method: A randomized, double-blind, placebo-controlled, parallel-group trial was conducted. Seventy healthy volunteers were enrolled and assigned into two groups receiving either placebo or LC-Plasma capsules (approximately 1 × 1011 cells/day) for 8 weeks. The skin microbiome was analyzed by NGS and qPCR. Gene expression was analyzed by qPCR and skin conditions were diagnosed by dermatologists before and after intervention. (3) Result: LC-Plasma supplementation prevented the decrease of Staphylococcus epidermidis and Staphylococcus pasteuri and overgrowth of Propionibacterium acnes. In addition, LC-Plasma supplementation suggested to increase the expression of antimicrobial peptide genes but not tight junction genes. Furthermore, the clinical scores of skin conditions were ameliorated by LC-Plasma supplementation. (4) Conclusions: Our findings provided the insights that the dietary supplementation of LC-Plasma might have stabilizing effects on seasonal change of skin microbiome and skin conditions in healthy subjects.

Highlights

Introduction published maps and institutional affilThe skin is the largest epithelial layer and serves as a body shield by preventing evaporation of body fluids and protecting from the harmful environmental substances [1,2].In addition to the skin’s own function as a first defense line against foreign substances, the skin is home to a diverse population of microbes
Since several skin disorders can be caused in case of the imbalance between commensal and pathogenic bacteria [8], the redundancy of skin microbiome is considered to be important to maintain the skin condition in healthy human
One subject in the placebo group and one subject in the LC-Plasma were excluded from analysis due to the low ingestion ratio

Summary

Introduction

In addition to the skin’s own function as a first defense line against foreign substances, the skin is home to a diverse population of microbes. The majority of these skin microbes are nonpathogenic commensals or transients [3]. Epidermal commensal bacteria play an essential roles to maintain the healthy skin by preventing the colonization of pathogenic bacteria [4], regulating skin pH [5], and contributing to the immune response [6,7]. Number of subjects p-Value Age 34.1 ± 7.2 34.0 ± 6.5 BMI 21.1 ± 2.9

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