THE EFFECTS OF DIETARY INCLUSION OF DETOXIFIED Leucaena leucocephala LEAF MEAL ON THYROIDAL ACTIVITY OF RATS DURING GESTATION-LACTATION PERIOD

Inna Narayani,Ngurah Intan Wiratmini,Ni Luh Eka Setiasih

doi:10.24843/ijbb.2018.v05.i02.p02

Inna Narayani, Ngurah Intan Wiratmini + Show 1 more

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https://doi.org/10.24843/ijbb.2018.v05.i02.p02

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Abstract

Leucaena leucocephala provides the highest amount of proteins compared to other greens. However, its use is restricted due to the content of mimosine, an antinutrition non-protein amino acid known to be toxic to animals. This study aimed to observe the effect of administration of more than 10% of processed Leucaena leucocephala leaf meal to the level of T3, T4 and the thyroid histopathology. In this study, Leucaena leucocephala leaves were soaked in distilled water for 12 hours. The processed of Leucaena leucocephala leaf meal was made into pellets containing 0%, 7.5%, 15%, and 22.5% leaf meal of total feeds respectively, and was fed to the rats during pregnancy and lactation. The level of triiodothyronine (T3) and thyroxine (T4), and the thyroid histological features were the parameters observed. The collected data were statistically analyzed by SPSS 20.0 for Windows using One-way ANOVA followed by Duncan Multiple Range Test to observe any significant difference among the 4 treatment groups (?=5%). Meanwhile, the presence of hyperplastic cells and follicle lumens filled with vesicles and colloids was descriptively analyzed by means of available literatures. The analysis of T3 and T4 level revealed that there was no any significant difference between the control and treatment groups. The epithelial cells of thyroid follicles in the treatment groups of P1, P2 and P3 showed hyperplasia and were detached from their respective basal membranes.

Highlights

Leucaena leucocephala (L.leucocephala) leaves contain proteins as high as 25-35% of their dry weight (Gosh and Bandyopadhyay, 2007)
This study aimed to observe the effect of administration of more than 10% of processed L. leucocephala leaf meal to the level of T3, T4 and the thyroid histopathology
The leaves were later milled and sifted to produce L. leucocephala leaf meal according to Zakayo et al, (2000) which made into pellets as such: The feeds used for the control group (P0) contained 0% of detoxified L. leucocephala leaf meal and consisted only of commercial pig feed CP 551, while the one used for treatment groups was a mix of CP 551 and detoxified L. leucocephala leaf meal designated as P1, P2, and P3

Summary

Introduction

Leucaena leucocephala (L.leucocephala) leaves contain proteins as high as 25-35% of their dry weight (Gosh and Bandyopadhyay, 2007). Additional 75% L. leucocephala leaf meal in cow’s feed for more than 22 days caused a decline in serum level of T3 (triiodothyronine) and T4 (thyroxine) (Gosh et al, 2007). Ly et al (2007) reported that excessive consumption of L. leucocephala by animals could lead to decrease in body weight, thyroid dysfunction, alopecia, and decline in both quality and quantity of. THE EFFECTS OF DIETARY INCLUSION OF DETOXIFIED Leucaena leucocephala LEAF MEAL ON THYROIDAL ACTIVITY OF RATS DURING GESTATION-LACTATION PERIOD Ngurah Intan Wiratmini, Inna Narayani, and Ni Luh Eka Setiasih semen, impairing reproductive function. Mimosine exhibits negative effects in ruminants and in monogastric animals, in which administration of more than 10% L. leucocephala leaf meal was conducted (Rai et al, 1992)

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