Abstract
Optimized doses and dosing strategies for trace element application in the anaerobic digestion (AD) process still face challenges. In this study, the response surface methodology (RSM) of Box-Behnken was used to investigate the effects of Fe, Co and Ni co-supplementation dosages on the mesophilic AD process of food waste (FW). According to RSM results, the maximum experimental methane yield of 677.9 mL/g VS was obtained under the optimal condition (Fe: 113.1, Co: 0.51, and Ni: 2.44 mg/L), representing a 27.4 % increase compared to the control group without trace element addition. The noticeable decrease in Fe bioavailability in control group indicates a higher demand for Fe during AD of FW. However, while increasing the Fe dose did not enhance its easily bioavailable fraction, that of Co and Ni exhibited a negative effect on Fe bioavailability. In the optimal condition, the syntrophic methanogenesis between syntrophic genera and obligate hydrogenotrophic methanogens improved, along with the increased relative abundance of ATP-binding cassette transporter and two-component systems, which correlated with enhanced Fe bioavailability. These findings provide deeper insights into the interactions of co-supplemented trace elements during AD of FW.
Published Version
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