The effects of COE on the expression of VEGF and CECs of Hepa1-6 tumor bearing mice

Abstract

Objective To study the effects ofCelastrus orbiculatus extracts （COE） on the expression of VEGF and CECs of Hepal-6 tumor bearing mice. Methods Animal Models of Hepal-6 portability liver tumor bearing mice were established and randomly assigned to six groups （eight mice per group） as follows： untreated control group, solvent vehicle control （DMSO） group, physiological saline control group, cisplatin-treated group, and different dosages COE-treated groups （40 rag＆g, 20 mg/kg, 10 mg/kg） , To approach the effects of COE on the expression of VEGF and CECs in Hepal-6 toumor bearing mice. Results The ELISA and flow cytometer showed that COE could significantly inhibit the expression of VEGF（45.24± 21.36） p g/ml and CECs （0.83±0.10） % compared with the physiological saline control group （P 〈 0.01 ） with the dose of 20mg/kg. Conclusion COE could significantly down-regulate the expression of VEGF and CECs. It may be related with the effect of COE in inhibiting angiogenesis of tumor. Key words: Celastrusorbiculatus; Angiogenesis; CECs; VEGF