The effect of mitofusin-2 gene on the neoplasia,proliferation and metastasis of human breast carci-noma cells in vivo

Abstract

Objective To investigate the effects of Mitofusin-2 gene (mfn2) on the neoplasia, proliferation and metastasis of Human breast carcinoma ceils in vivo. Methods The cells were divided in-to three groups:non-transfection group as negative control group, pEGFP-C2 transfection group as experi-mental control group, and pEGFPmfn2 transfection group as experimental group. Fifteen nude mice were randomly divided into 3 pups:the experimental group, the experimental control group, and the negative groups that were heterografted with three groups' cells respectively. The expression of mfn2 was detected by RT-PCR in tumor tissues. The expression of Ki-67 and VEGF was detected by immunohistochemistry. Results The tumor weight in negative control, experimental control and experimental groups was (2.77± 0.12), (2.84±0.16) and (1.78±0.13) g, respectively. The tumor weight in experimental group was significantly lighter than in two control groups (P<0.05). The tumor volume in experimental group was markedly less than in experimental control and negative control groups (P<0.05). The expression of mfn2 in experimental group was higher than in experimental control and negative control groups (P< 0.05). The expression of Ki-67 in experimental group was higher than in experimental control and negative control groups (P<0.05),but the expression of VEGF in experimental group was lower than in experi-mental control and negative control groups (P<0.05). Conclusion Exogenous mfn2 can strongly inhibit proliferation and metastatic progression of breast carcinoma MCF-7 in xenograft implantation model. Key words: Mitofusin-2 gane; Neoplasia; Gene therapy