Effects of microRNA-194 on proliferation, migration, invasion and epithelial- mesenchymal transition of basal- like breast cancer cell line HCC1937

Abstract

Objective To explore the role of microRNA- 194(miR- 194)in proliferation, migration,invasion and the epithelial- mesenchymal transition(EMT)of human basal- like breast cancer cell line HCC1937. Methods Three groups were set up:experimental group[HCC1937 with lentivirus- miR - 194- enhanced green fluorescent protein(EGFP), MOI= 50], negative control group(HCC1937 with lentivirus- CON157- EGFP), and blank control group(HCC1937 without lentivirus). The miR- 194 expression was detected by real- time quantitative reverse transcriptase- polymerase chain reaction(RT-qPCR). Cell growth ability of HCC1937 was determined by cell counting kit- 8(CCK- 8)assay. Cell migration and invasion ability of HCC1937 were mearsured by a Transwell assay, and the protein of epithelial - mesenchymal transition phenotype was measured by Western blotting. Results The expression of miR-194 in experimental group(432.93±0.01) was higher than in negative control group(0.90±0.02) and blank control group(1)(P< 0.01) The cells in experimental group grew more slowly[(44.81± 0.11)%]than negative control group[(104.29±0.13)%]and blank control group[(100±0)%, P< 0.01].The number of migration and invasion was both less in experimental group than in negative control group and blank control group(P< 0.05).Western blotting revealed that the expression of E- cadherin in experimental group(0.175±0.053) was significantly decreased as compared with negative control group (0.516±0.032)and blank control group(0.433±0.047, P< 0.05), and the expression of N- cadherin in experimental group(0.342±0.012)was significantly increased as compared with negative control group (0.137±0.007)and blank control group(0.142±0.021)(P<0.05) Conclusion miR- 194 can inhibit proliferation, migration and invasion of basal- like breast cancer cells(HCC1937), and also affect EMT process in HCC1937 cells. Key words: Basal- like breast cancer; MicroRNA- 194; Proliferation