Abstract
The purpose of this study was to evaluate the effects of alginate composition within microcapsules on the viability, gene expression, and albumin secretion of encapsulated hepatocarcinoma cells (HepG2). Two compositions were tested: a high guluronic acid content (64% in G) and a high mannuronic acid content (67% in M). In both alginates, HepG2 cells formed multi-cellular aggregates which exhibited a high degree of cell–cell contacts. Compared with cells encapsulated in alginates with a high M content, HepG2 cells encapsulated in alginates with a high G content experienced a low speed of proliferation and viability due to the growth inhibition of the alginate matrix. Our data also demonstrated that an increasing in the content of high G content reduced the albumin secretion and genes expression involved in liver-specific functions. These observations indicated that alginates with a high M content were favorable matrix used for liver tissue engineering.
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