Abstract
The aim of the present study is to determine the efficiency of silver nanoparticles in mastitis treatment induced by Staphylococcus aureus, 20 lactating goats were infected intramammaryinoculated with 1.5×108 cfu/ml of virulence Staphylococcus aureus isolated from milk of caprine mastitis and at 48hr post inoculation, the animals were divided randomly into four groups equally, 1st group left without treatment as control positive, 2nd group treated intramammaryinfusion with 5 ml Silver nanoparticle after 2h from infection daily for 3 consecutive days, 3rd group treated intramammaryinfusion with 5 ml Silver nanoparticle after 72h from infection daily for 3 consecutive days, 4th group treated intramammaryinfusion with 5 ml Silver nanoparticle mixed with gentamycin after 72h from infection daily for 3 consecutive days. The silver nanoparticles used in this study were created using a green method from Bacillus clausii. The Scanning electron microscope was spherical and homogeneous form, with a size range of 25 to 45 nm. Results showed severe clinical signs of the mammary gland with heavy bacterial isolation in 1st control groups at 3, 6-, 9-, 14- and 21-days post infection, while animals that were intramammary treated with Silver nanoparticle alone or combination with gentamycin showed a disappearance in clinical signs of the mammary gland and bacterial isolation, it was concluded that Silver nanoparticle had a strong action against mastitis caused by Staphylococcus aureus.
Highlights
Mastitis is an infection of the mammary gland parenchyma that may inflict partial or total damage to the gland
The aim of the present study is to determine the efficiency of silver nanoparticles in mastitis treatment induced by Staphylococcus aureus, 20 lactating goats were infected intramammary inoculated with 1.5×108 cfu/ml of virulence Staphylococcus aureus isolated from milk of caprine mastitis and at 48hr post inoculation, the animals were divided randomly into four groups 1st group left without treatment as control positive, 2nd group treated intramammary infusion with 5 ml Silver nanoparticle after 2h from infection daily for 3 consecutive days, 3rd group treated intramammary infusion with 5 ml Silver nanoparticle after 72h from infection daily for 3 consecutive days, 4th group treated intramammary infusion with 5 ml Silver nanoparticle mixed with gentamycin after 72h from infection daily for 3 consecutive days
Results showed severe clinical signs of the mammary gland with heavy bacterial isolation in 1st control groups at 3, 6, 9, 14- and 21-days post infection, while animals that were intramammary treated with Silver nanoparticle alone or combination with gentamycin showed a disappearance in clinical signs of the mammary gland and bacterial isolation, it was concluded that Silver nanoparticle had a strong action against mastitis caused by Staphylococcus aureus
Summary
Mastitis is an infection of the mammary gland parenchyma that may inflict partial or total damage to the gland. Several pathogens can cause mastitis, but S. aureus is the most commonly diagnosed causal microorganism of intra mammary infection (IMI) in goats. S. aureus infection in the mammary gland warrants particular consideration due to the carriage of various virulence factors for this bacterium causes both acute CM (gangrenous mastitis) and SCM in lactating goats [2]. The ability of S. aureus to produce extremely toxic compounds that damage mammary gland membranes. Intramammary infection caused by S. aureus in dry period persists during lactation and may pose a potential risk to human health because of the risk of transmission of pathogens, and because of the presence of enterotoxins in milk or milk products especially in goats' milk [3]
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