Abstract

The work is devoted to the assessment of survival of nodule lupine bacteria ( Rhizobium lupini ) on seeds and to the study of the opportunities to increase the terms between seed inoculation and their sowing. It has been evaluated the effect of such water-soluble polymers as sodium alginate, carboxymethyl cellulose, polyvinyl alcohol (4-88, 4-98) and polyvinylpyrrolidone on survival of nodule lupine bacteria. The number of surviving nodule bacteria of lupine R. lupini (str. 363a and 367a) was determined on inoculated lupine seeds of the variety ‘Oligarkh’ after 24 hours after inoculation with a control working solution (20% bacterial suspension in water), as well as with the solutions modified with five percent of such water-soluble polymers as sodium alginate, carboxymethylcellulose (CMC), polyvinyl alcohol of 2 grades (PVA 4-88 and PVA 4-98) and polyvinylpyrrolidone (PVP). The experiment has identified PVP as the most effective polymeric rhizobium protector among all above-mentioned ones because the number of surviving bacteria in the PVP variant was significantly larger than the control variant and in 2–3 times larger than in the variants with other polymers. There has been studied dynamics of bacteria death from the moment of inoculation and after 2, 4, 8, 24, 48 and 168 hours in various variants of the experiment. The effectiveness of PVP as a rhizobium protector on inoculated seeds was determined, its optimum concentration was identified.

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