The effect of various concentration of quail egg yolk on spermatozoa motility of kancra fish (Tor soro Valenciennes, 1842) post cryopreservation

Abstract

Kancra fish, also known as Batak fish, is often used in North Sumatera in traditional ceremonies. Nevertheless, overfishing has led to a decrease in its population. Cryopreservation is a strategy that might be effectively used to solve this problem. This study aimed to evaluate the effect of various concentrations of quail egg yolk on the spermatozoa motility of kancra fish after 48-hours of cryopreservation. The concentration of quail egg yolk were 0%, 5%, 10%, 15%, 20%, and 25%. The sperm of kancra fish were collected using the stripping method and was diluted with the quail egg yolk, fish ringer, and 10% methanol. It was then equilibrated for 10 minutes at a temperature of 5° C before being frozen at -10 °C for 48 hours. Thawing was carried out at 40 °C for 1 minute. The motility was analyzed using ANOVA and proceeded by Tukey test. The ANOVA test showed the use of various concentrations of quail egg yolk has a significant effect (p<0.05) on the spermatozoa motility of kancra fish after cryopreservation. The concentration of 10% of quail egg yolk showed the highest spermatozoa motility (85.10 ± 1.51%) after 48-hours of cryopreservation (p<0.05).