Cryopreservation of java barb (barbonymus gonionotus) using egg yolk as a cryoprotectant

Abstract

The aim of this study was to inversitage the effect of egg yolk as a cryoprotectant on spermatozoa quality of Barbonymus gonionotus, twenty four hours after sub-zero freezing. The ejaculates from a total of three males were diluted with the solvent (glucose-base extender + 10% methanol).Egg yolk concentration which was used in this study were: 0%, 5%, 7%, 9%, 11%, 13%, 15% and 17%, respectively. Samples were then equilibrated at 4°C for 10 minutes and were freezed at-34°C for 24 hours. Thawing was carried out at 40°C for 30 seconds. Based on Anova test, there were significant effect (P<0.01) of various concentration of egg yolk on post-thaw sperm motility and viability, but not on post-thaw abnormality, compared to control (0% of egg yolk). According to the Tukey test, the concentration of 15% of egg yolk showed significant difference (P<0.01) on post-thaw motility and viability, respectively. Fifteen percent of egg yolk showed the highest post-thaw sperm motility (96.10±3.31) and sperm viability (85.50±3.11), respectively. The successful cryopreservation were influenced by concentration used in this study were: 0%, 5%, 7%, 9%, cryoprotectant and extender. Some methodologies, 11%, 13%, 15% and 17%, respectively. development and application of cryopreservation of fish spermatozoa were reported for species: Osphronemus Equilibration and Freezing: Samples were then goramy (3-5), Barbonymus gonionotus (6), carp (7-9), equilibrated at 4°C for 10 minutes and were freezed at-34°C rainbow trout (10) and other salmonids (11). The objective for 24 hours. of present study is to investigate the effect of egg yolk in various concentration of 0%, 5%, 7%, 9%, 11%, 13%, Post-thaw Parameters Examined: Thawing was carried 15% and 17%, respectively, on spermatozoa quality of out at 40°C for 30 seconds. After thawing, each sample Barbonymus gonionotus Bleeker, 1850 cryopreserved for was then evaluated for the following parameters using a 24 hours light microscope with the aid of a digital eye-piece