Abstract
Objective To study the effect and possible mechanisms of mesenchymal stem cells （MSCs） transplantion therapy mediated by ultrasound in combination with nitric oxide （NO） microbubbles （MBs）on cardiac function in rats with myocardial infarction （MI）. Methods Twenty-eight rats with MI were randomly divided into the following groups by use of random digits table： phosphate buffered saline group （ injection of PBS into the tail vein） , MSCs group （injection of MSCs into the tail vein） , ultrasound ＋ MBs ＋ MSCs group （ultrasound interven- tion when injection of ordinary MBs into the tail vein followed by injection of MSCs） and ultrasound ＋ NO MBs ＋ MSCs group （ultrasound intervention when injecting NO-MBs into the tail vein followed by injection of MSCs） （n = 7, each group）. After four weeks, the left ventricular systolic function was evaluated with M-mode ultrasound for each group, capillaries density of myocardial ischemic area was counted in each group, and the expression of vascular endothelial growth factor （VEGF） was detected by Western blot and real time PCR. Results The ejection fraction （EF） of NO-MBs group was significantly higher than the other groups （ P 〈 0.05 ）. The number of capillaries in NO- MBs group was also much more than that in the other groups （P 〈 0.05）. The expression of VEGF in infarcted zone was much higher in the NO-MBs group than that in the other groups （ P 〈 0.05 ）. Conclusion Ultrasound and NO MBs-mediated MSCs transplantation therapy could improve the cardiac function of rats after MI, and the possible mechanism was the upregulation of VEGF and angiogenesis. Key words: Myocardial infarction ; Stem cells ; Ultrasound ; Microbubbles agent ; Nitric oxide
Published Version
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