The effect of Tripterygium Wilfordii Hook. F. and Tripterygium Hypoglaucum(Lévl.)Hutch on macrophage inflammatory factor

Abstract

Objective To observe the effect of Tripterygium Wilfordii Hook. F. and Tripterygium Hypoglaucum(Levl.)Hutch on macrophage inflammatory factor, and to provide the oretical basis and experimental basis for the clinical application of these drugs. Methods Two batches of Tripterygium Wilfordii Hook. F. and Tripterygium Hypoglaucum(Levl.)Hutch were collected, and then the samples turned into alcohol extract by extraction and isolation. The IC50 values of alcohol extracts were measured by MTT in BMDM cell. BMDM cell induced by the 4 batches of samples with IC50, then IL-6, IL-10, iNOS were detected by Elisa. Results The content of IL-6(5.08 ± 0.96 pg/ml, 6.24 ± 0.20 pg/ml vs. 7.92 ± 0.84 pg/ml)and iNOS(0.14 ± 0.04 ng/ml, 0.36 ± 0.11 ng/ml vs. 0.86 ± 0.13 ng/ml)in Anhui and Guizhou groups were significantly lower than sulfasalazine(P<0.05), and the content of IL-10(21.20 ± 4.24 pg/ml, 26.49 ± 4.44 pg/ml vs. 9.06 ± 0.40 pg/ml)in Anhui and Guizhou groups were significantly higher than sulfasalazine(P<0.05). The content of IL-6(4.22 ± 0.38 pg/ml, 4.55 ± 0.44 pg/ml vs. 7.92 ± 0.84 pg/ml)and iNOS(0.07 ± 0.04 ng/ml, 0.28 ± 0.10 ng/ml vs. 0.86 ± 0.13 ng/ml)in Hunan and Zhejiang groups were significantly lower than sulfasalazine(P<0.05). Conclusion The anti-inflammatory effect of Tripterygium Wilfordii Hook. F. treat rheumatoid arthritis is better than sulfasalazine and Tripterygium Hypoglaucum(Levl.)Hutch. Key words: Tripterygium Wilfordii Hook. F.; Macrophages; Arthritis, rheumatoid; Inflammation