THE EFFECT OF TOPICALLY USING MELATONIN AND ANABASIS ARTICULATE EXTRACT WITH VAGINAL SPONGES ON SOME HORMONES AND ANTIOXIDANT STATUS

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This study was conducted in the University of Anbar College of Agriculture in the Department of Animal Production Sheep Field, for the period from 10/15/2021 to 5/7/2022. The aim of this study is to use the melatonin hormone in two different doses and the Anabasis articulate extract in vaginal sponges topically, to understand their effectiveness on the oxidative state and some of the reproductive hormones on the local Iraqi ewes. The study was conducted on twenty local ewes within 4-2.5 years age range and 47 kg weight average. All ewes were treated with vaginal sponges for 14 days, and was randomly divided into four treatment groups: (T1) 20 mg melatonin hormone, (T2) 30mg melatonin hormone, (T3) Anabasis articulate extract at a concentration 3 g and (T4) was left without any treatment and was considered a control treatment. The treatment was applied through a sponge with a hole made in it using a surgical scalpel; that hole position should be away from a pulling thread attached to the sponge to avoid cutting it accidentally and thus not being able to pull the sponge out. The treatment continued from day 0 of pushing the vaginal sponges until day 14 when they were withdrawn. All ewes were injected with eCG hormone at a dose of 500 IU intramuscularly after sponge withdrawal. Also, the experimental ewes were kept with 3 rams for 3 days. Blood samples were collected from the jugular vein and the levels of estrogen, progesterone, antioxidants glutathione peroxides (GPX), and Malone Dialdehyde (MDA) were measured. The experiment treatments showed no significant differences in both estrogen and progesterone hormone levels compared with the control group, which indicates that there was no effect of the sponge materials on the hormones absorption. As for the antioxidants, the study showed the superiority of the experimental treatments in the levels of GSH compared to the control group. Also, the MDA recorded a decrease in its levels in the treated groups compared to the control one. We conclude from this study that placing the hormone melatonin at 20,30 Mg concentration and the Anabasis extract at 3g in vaginal sponges did not affect the hormone’s actions neither its absorption from the sponge, but rather improved the uterus conditions and prevent fluid accumulation around the sponge. It also prevents infections and improves the oxidation state by increasing GSH activity and MDA level reduction.