Abstract
Purpose: Magnetic field is one of the effective and non-invasive modalities on biology and angiogenesis. Studies on the effects of magnetic fields on angiogenesis showed that the shape of the magnetic field could potentially affect angiogenesis. Therefore, this study aimed to control the frequency, intensity, and duration of exposure of magnetic field while investigating the effect of the shape of the magnetic field on the viability of Human Umbilical Vein Endothelial Cells (HUVECs).
 Materials and Methods: The HUVECs were exposed to various shapes of 50 and 60 Hz magnetic fields with intensities of 0.5 and 1 mT in acute and chronic exposure regimes. The viability of HUVECs was assessed via MTT assay.
 Results: Results showed that the sin type 50 and 60 Hz magnetic fields are more effective in decreasing the viability. The rectified 100 and 120 Hz with 1 and 0.5 mT could increase and decrease the viability compared with 50 and 60 Hz, respectively.
 Conclusion: It can be concluded that the shape of the magnetic field can be an effective factor in biology and must be controlled to have a reliable response.
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