The effect of surfactant micellization on the cytotoxicity of silver nanoparticles stabilized with aerosol-OT

Abstract

The toxic action of surfactant used as a stabilizer of metal nanoparticles have been studied with the aim to determine separate contributions of surfactant monomers and micelles to cell viability decrease. Basing on (1) the well-known ability of surfactant molecules to form micelles in solution at a critical micelle concentration (CMC) and (2) the results reported in literature, showing that toxicity of various surfactants increases when their concentration exceeds CMC, we supposed that surfactant molecules and micelles may differ in their toxic effect on cells. This supposition was verified on the anionic surfactant aerosol-OT (AOT) used as a stabilizer of silver nanoparticles (AgNPs) in studies of their cytotoxicity on Jurkat cells by means of the MTT test. Two samples of AgNPs stabilized with AOT in concentrations higher (3 mM) and lower (1 mM) than its CMC in water were introduced to the cell medium as water solutions diluted to obtain nanoparticle concentrations in the range 1–7 μg/mL. Cell viability changes were registered after 24 h incubation. It was found that AgNPs of similar average size (about 16 nm), synthesized by the same procedure, introduced to the same concentrations in cell medium, produced a different effect on cell viability. Namely, decrease in cell viability was observed for AgNPs with 3 mM AOT, while no noticeable changes were registered for AgNPs with 1 mM AOT. A similar difference was detected for the corresponding dilutions of 3 mM and 1 mM AOT water solutions. We assumed that the toxicity dependence on AOT concentration originated from the difference in toxic action of the two different AOT forms – molecules (monomers) and micelles – present in the AgNPs and AOT solution. The approach was suggested for estimation of the separate contributions of monomers and micelles to the total AOT toxicity; changes of these contributions with AgNPs or AOT concentration were also determined. The results obtained may prove to be useful in studies of the biological activity of surfactants applied both as nanoparticle stabilizers and as agents working in medicine as suppressors of various infections.