The effect of sucrose concentrations and shoot inoculum density on the growth of in vitro shoot of teak (Tectona grandis L.)

Abstract

The teak belongs to the highly prized timber tree. Quality teak trees have been guaranteed by planting clonal planting materials propagated vegetatively, for example, by shoot tip cutting and tissue culture. Tissue culture propagation of teak has been commercially viable even though the efficiency of production can still be improved. This research aimed to study the effect of increasing shoot inoculum density and sucrose concentration requirement for optimum growth of teak shoot in vitro. Inocula of a single nodal shoot in the number of 5 or 9 were inoculated on modified MS basal media with sucrose concentrations treatment of 20, 30, 40, 50, and 60 g l−1. The results showed that the standard sucrose concentration of 30 g l−1 and a higher concentration of 40 g l−1were optimum for the growth the inocula of the densities tested. Even though the average shoot growth was lower for nine inocula than that of five inocula, the total shoot growth was higher for the former. An increasing number of inocula can be used as a method to increase the efficiency of shoot proliferation stage of teak micropropagation.