Effect of sucrose concentrations on in vitro growth and subsequent acclimatization of the native bromeliad Vriesea inflata (Wawra) Wawra

Camila Freitas,Victória Carvalho,Catarina Carvalho Nievola

doi:10.5007/2175-7925.2015v28n3p37

Camila Freitas, Victória Carvalho + Show 1 more

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https://doi.org/10.5007/2175-7925.2015v28n3p37

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Journal: Biotemas	Publication Date: Jun 16, 2015
Citations: 5	License type: cc-by

Affiliation: Instituto de Botânica

Abstract

Using sucrose is crucial for in vitro plant growth, due to decreased photosynthesis under this condition. However, ornamental epiphytic species, which have slow growth, have shown better development in nutrient media with low concentrations of this carbohydrate. Vriesea inflata (Wawra) Wawra is an epiphytic bromeliad native to the threatened Atlantic Rain Forest and it has a high ornamental value. This study aimed to evaluate the effects of various sucrose concentrations in the culture medium on in vitro growth and subsequent ex vitro acclimatization. Plants were cultured in vitro in basal media without carbohydrates; and in media containing 1.0; 1.5; 3.0; 4.5; and 6.0% (w/v) of sucrose for 60 days. After, plants were acclimatized for 90 days. Plants cultured in sucrose concentrations over 3.0% had the smallest development after in vitro culture and acclimatization. Nevertheless, those cultured at lower concentrations had the highest in vitro root growth, and they generated larger plants after acclimatization. On average, 1.7% sucrose was defined as appropriate to cultivate V. inflata in vitro , in order to obtain more vigorous plants during acclimatization.

Highlights

The bromeliad Vriesea inlata (Wawra) Wawra is native to the threatened Atlantic Rain Forest, and it has morphological traits of high ornamental value (PEDROSO et al, 2010) (Figures 1a; 1b)
This species and many other bromeliads are extracted from their original habitat to supply the ornamental trade, something which can pose a signiicant threat to the Bromeliaceae biodiversity (NUNES, 2002; NEGRELLE et al, 2012)
This study aimed to evaluate the effects of various sucrose concentrations on in vitro and subsequent ex vitro V. inlata growth, in order to verify if lower concentrations improve the species’ acclimatization

Summary

Introduction

The bromeliad Vriesea inlata (Wawra) Wawra is native to the threatened Atlantic Rain Forest, and it has morphological traits of high ornamental value (PEDROSO et al, 2010) (Figures 1a; 1b). This species and many other bromeliads are extracted from their original habitat to supply the ornamental trade, something which can pose a signiicant threat to the Bromeliaceae biodiversity (NUNES, 2002; NEGRELLE et al, 2012). The development of plants in vitro depends to a large extent on the supply of carbon sources in the culture media, since morphogenesis and growth are high energy requiring processes. Bromeliads are mostly cultured in vitro with 3% sucrose in the medium (DAL VESCO et al, 2011; CARVALHO et al, 2013; MARTINS et al, 2013)

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