The effect of storage time on the whole blood (WB) quality at the blood bank of Dr. Soetomo general hospital

Abstract

Several changes occur during whole blood (WB) storage in the blood banks. The structure of erythrocytes and thrombocytes changes, and their viability decreases. Leukocyte degradation causes the release of cytokines and enzymes that can trigger a transfusion reaction. In addition, a decrease in pH will damage the WB components. The coagulation factors will be degraded during the storage process. All of these changes will impact the WB quality and the recipient. Therefore, this study aims to analyze the effect of storage time on WB quality. This research employed an analytical study with a time series design conducted at the Clinical Pathology Installation and Blood Bank at Dr. Soetomo General Hospital, Surabaya, on February-June 2020. The researchers utilized a sample of sixteen WB bags. The sample will be tested with several examinations, including a complete blood count examination (to determine the number of erythrocytes, leukocytes, thrombocytes, and hemoglobin), the coagulation examination (to determine PPT and APTT), and a BGA examination (to determine pH, pO2, and pCO2). All parameters were checked on day 0, day 5, day 10, day 20, and day 30.