The Effect of Storage on Full Blood Count in Different Anticoagulant

Abstract

The effect of storage on full blood count in different anticoagulant was determined in view of it's importance on the reliability and validity of test results. The study was designed to know the effect of storage on full blood count in different anticoagulant which is Ethylendiamine tetra acetic acid and citrate phosphate dextrose Adenine. A total of 50 samples of apparently healthy individuals were analyzed for their packed cell volume, Haemoglobin concentration, Red blood cell, Platelet, white blood cell and differential Neutrophils, Lymphocytes and monocytes, by storing 2mls each of their blood sample in Ethylendiamine tetra acetic acid and Citrate phosphate dextrose adenine (CPDA) anticoagulant for a period of 24hrs and 168hours at 4 0 C. Changes were observed in some but not all haematological parameters measured on LDW-3600 Auto haematological analyzer, specifically the HB, PCV, Lymphocytes and Monocytes showed a drastic increase on day seven compared to day one and more decreased on platelet and WBC on seven day compared to day one. For this study no statistical change was observed for Rbc. Based on these findings, samples stored for 24hours at 4 0 C would not result in significant changes in blood parameters, therefore haematological laboratories are advised not to keep samples beyond 24hours at 4 0 C for reliability of test result.