Abstract
Aim:The effect of citrate phosphate dextrose adenine-1 on the hematological parameters of stored Nigerian indigenous dog’s blood with plastic vacutainer was investigated. This was done in view of determining the viability and stability of the studied parameters for blood banking purpose. Till date, there is no literature on the stability of whole blood of Nigerian indigenous dogs for blood banking purposes.Materials and Methods:A total of six apparently adult healthy dogs were sampled, and their blood was stored at 4°C and analyzed for their packed cell volume (PCV), hemoglobin (Hgb) concentration, red blood cell (RBC) count, total and differential white blood cell (WBC) count, platelet count (PC), mean corpuscular values (mean corpuscular Hgb [MCH], MCH concentration, and mean corpuscular volume [MCV]), blood potency of hydrogen (pH), and erythrocyte sedimentation rate (ESR) over a period of 14 days.Results:Significant changes were observed in some of the studied parameters. Of the 14 days’ study period, PCV, Hgb concentration, total WBC count, PC, and neutrophil count showed no significant (p≥0.05) difference until day 2 post-storage (PS), while RBC count, ESR, MCV, and lymphocyte count showed no significant (p≥0.05) variation up until day 3 PS. Blood pH showed no significant (p≥0.05) variation within 24-h PS but was significantly (p≤0.05) higher than obtained values on days 1, 3, 7, 10, and 14 PS.Conclusion:Based on our finding, we could suggest that hematological laboratories and hemotherapists could use canine blood stored in a plastic vacutainer for 2-3 days.
Highlights
Whole blood degrades progressively during the weeks of refrigerated storage [1]
Materials and Methods: A total of six apparently adult healthy dogs were sampled, and their blood was stored at 4°C and analyzed for their packed cell volume (PCV), hemoglobin (Hgb) concentration, red blood cell (RBC) count, total and differential white blood cell (WBC) count, platelet count (PC), mean corpuscular values, blood potency of hydrogen, and erythrocyte sedimentation rate (ESR) over a period of 14 days
Based on our finding, we could suggest that hematological laboratories and hemotherapists could use canine blood stored in a plastic vacutainer for 2-3 days
Summary
Hematological, biochemical, and physical properties of whole blood are adversely affected during storage by preservatives used, storage temperature, containers used, and frequency of gentle mixing during storage [2]. While practices vary from country to country, preservative solutions permitting shelf life as long as 7 weeks have been licensed [3]. These preservative solutions such as citrate phosphate dextrose adenine-1 (CPDA-1) are often contained in blood bags meant for the collection of blood for transfusion. It has been documented that blood stored in CPDA-1 anticoagulated bags remained stable for up to 35 days [1,3].
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