The effect of sperm morphology and sperm acrosin activity on fertilization failure in vitro fertilization

Abstract

Objectives To analyze the relationship between morphology and acrosin activity of sperm and fertilization failure of in–vitro fertilization(IVF) . Methods 982 patients underwent IVF were analyzed retrospectively in 2014.Semen samples were analyzed by the criteria of the 5th edition of the WHO Laboratory Manual for the Examination and Processing of Human Semen(WHO5). The percentages of normal morphology(x) were divided into five groups: group one: x≥4%, group two: x<1%, group three: 1%≤x<2%, group four: 2%≤x<3%, group five: 3%≤x<4%, and then were divided into nomal and bnormal groups by sperm acrosin activity. Results There was significant difference in the normal sperm morphology and acrosin activity between in–vitro fertilization failure group and normal group(P 0.05). There was significant difference in the rate of fertilization failure between the abnormal acrosin activity group and the normal group(P<0.01). Conclusions The abnormal of sperm morphology and sperm acrosin activity would increase the rate of fertilization failure of in–vitro fertilization. Key words: Insemination, Artificial; Spermatozoa; Acrosin