Abstract
The effects of formaldehyde, betapropiolactone (BPL), acetylethylenimine (AEI), and a deprived ionic environment on haemagglutinin (HA) and neuraminidase (NA) bound to PIV-3 were tested. Formalin elicited an increased NA activity and a significant decrease in the HA titres on three PIV-3 strains tested. The formalin-treated PIV-3 could be used in the neuraminidase inhibition (NI) test. The increased NA activity was due to an increased stability of the enzyme active sites of PIV-3. AEI and BPL did not affect the HA, but BPL caused an increased NA activity of a neuraminidase-weak strain. Following dialysis of PIV-3 against distilled water, an increased affinity of the virus-bound NA to the substrate sialolactose was found. The infectivity titres of PIV-3 were not altered by the dialysis, and virus preparations treated in this manner could be used in the NI-test. UV-treatment of PIV-3 resulted in a loss of infectivity and a loss of HA and NA activities.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
