Abstract
It is known that the cellulose layers in cotton fibers are made up of filament-like units termed fibrils. With the advent of electron microscopical techniques, an extensive effort has been put forth to arrive at a size for these units. The currently accepted value for the diameter of these elementary fibrils is approximately 35 A. U.In this report an indirect staining technique for electron microscopy has been employed to study the effect of mercerizing strength sodium hydroxide on the size of the elementary fibrils in cotton fibers. The method involves the addition of an unsaturated carbon-carbon double bond to the cellulose within the fibril and further reacting the double bond with osmium tetroxide. The compound used in this investigation was acrylic acid which not only has the osmium reactive bond but, because of its short molecular length, allows the osmium to situate itself at a close proximity to the structure under study.
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Schedule a callMore From: Proceedings, annual meeting, Electron Microscopy Society of America
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