The effect of sodium chloride and temperature on the levels of transcriptional expression of staphylococcal enterotoxin genes in Staphylococcus aureus isolates from broiler carcasses.

Abstract

Staphylococcus aureus is one of the most common pathogens associated with food poisoning, which is caused by the ingestion of food contaminated with staphylococcal enterotoxins (SE). Our study aims at evaluating the occurrence and expression of five SE genes (sea, seb, sec, sed, and see) in S. aureus previously isolated from broiler carcasses. Besides that, it also presents an in vitro analysis of the effects of sodium chloride and temperature on the levels of transcriptional expression. A total of 30 S. aureus isolates were investigated for the presence of SEs by PCR assay. The expression level and the effects of sodium chloride (2.5% NaCl), as well as temperature (8 ºC and 12 ºC), on the transcriptional expression, were evaluated by a quantitative reverse transcription PCR (RT-qPCR). Twelve isolates carried at least one of the SE genes. Among them, five representative isolates presented transcriptional expression for at least one gene. Both sodium chloride and low temperatures interfered with the expression of the SE genes, decreasing their values. However, one isolate displayed relative expression 2.25 times higher for sed gene than S. aureus FRI 361 in optimal conditions (p < 0.05), demonstrating their toxigenic potential even under salt stress. There was no evidence of enterotoxin gene expression at 8 ºC.