Abstract
：Objective To investigatethe expression of Oct4 in liver cancer, and the interrelation of the Oct4 and Wnt/β-cateningenes in hepatocellular carcinoma( HCC) cell line HepG2. Methods RTPCR technique was usedto detect the expression of Oct4 and β-Catenin in HCC specimens;RNAi was used to knock-down the expression of Oct4 in HepG2, and the change of Wnt/β-cateninrelated genes were detected by Real time-PCR. Results In HCC specimens, the expression ofOct4 and β-Catenin in tumor and cirrhotic liver tissues were stronger thannormal liver tissues. In SiRNA Oct4 HepG2 cells, the expression of Oct4 was downregulated,and β-cateninas well as Wnt10b were in a positive correlation with Oct4, TCF3 was in negativecorrelation with Oct4. Clone formation and move ability of the HepG2 were downregulated.Conclusions The expression of Oct4 was higher in tumor tissues than in normal livertissues. Silencing Oct4 by SiRNA-0ct4 in HepG2 resulted in decreased ability of cloneformation and cell movement. Key words: Carcinoma, hepatocellular; RNA interference; Oct4; Wnt/p-catenin signal pathway
Published Version
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