The effect of sikkam (Bischofia javanica) leaf extract on blood sugar levels and islet of langerhans in alloxan-induced diabetic rats

Abstract

Diabetes Mellitus is a metabolic disease characterized by hyperglycemia, occurring due to abnormal insulin secretion or insulin action. Alloxan induces diabetes by damaging pancreatic cells and initiating hyperglycemia. One of the plants that has the potential to decrease blood sugar is leaves of sikkam (Bischofia javanica), because it contains quercetin and gallic acid compounds which are classified as an antioxidant group. The objectives of this study were (1) to analyze the effect of sikkam leaf extract on the reduction in blood sugar levels of Wistar rats and improved the histology of langerhans islet and (2) determine the dose of sikkam leaf extract which has the most effective effect in reducing rat blood sugar levels. The design used is a non factorial complete random design. The samples used were 30 Wistar rats which were divided into 6 treatment groups and 5 repetitions, namely K0 (negative control), K1 (positive control), K2 (dose of ethanol extract of sikkam leaf (EESL) 300 mg/Kg BW), K3 (EESL 600 mg/kg BW), and K4 (EESL treatment 900 mg/kg kg) and K5 (Glibenclamid 4.5 mg). Data analysis used One-way ANOVA followed by LSD test. The results showed that there was an EESL effect on the reduction in rat blood sugar levels and improved the histology of Langerhans islet. EESL which has the best antidiabetic effect is on administering a dose of 900 mg/kg BW.