Abstract
A polymeric crosslinker poly(ethylene glycol) derivative with dioleyl groups as hydrophobic group at both ends was developed for promoting cellular spheroid formation. Our approach to bridge cells was based on the crosslinking of cell membrane using a crosslinker via the hydrophobic interaction. Using the crosslinker, spheroid formation of pancreatic β-cell line RIN in a round bottom 96-well plate was evaluated, especially in effect of serum on spheroid formation. In the presence or absence of serum, the size of prepared spheroid was found to decrease with increasing culture time and with increasing crosslinker concentration. However, the medium without serum proved to be a favorable condition for promoting cell aggregation because in this case spheroid with smaller size could be obtained. It was clarified that spheroid formation and insulin secretion of the spheroid prepared by the crosslinker were enhanced, especially in the medium without serum.
Highlights
Tissue engineering is an emerging technology that can be used to repair and regenerate damaged human tissue [1,2,3]
The number of cells remarkably decreased at the condition of 25 mg/mL crosslinker concentrations. These results suggested that the range of crosslinker concentration from 0 to 2.5 mg/mL presented the best condition for spheroid formation and its viability
Our approach to form spheroids was based on the crosslinking of cell membrane using a polymeric crosslinker via hydrophobic interaction
Summary
Tissue engineering is an emerging technology that can be used to repair and regenerate damaged human tissue [1,2,3]. The technique of tissue engineering has taken a lead role in producing new tissue using materials and cells. Cells are commonly cultured in vitro as monolayers in plastic flasks. It is well-known that there is a significant difference between cells lying within flat layer (2D culture) and cells embedded in three-dimensional tissue (3D culture) [48]. The function of cells in 3D culture generally escalates because of the enhanced cell-cell interaction. The cells in a spheroid are known to possess enhanced functions as compared to those in case of isolated cells; spheroid has great potential for its application in the field of tissue engineering
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