Abstract
Key messageTissue cultures are the only effective way to proliferate this valuable species (Daphne mezereum). These researches may help in solving the problem of reproduction of other woody plants.Daphne mezereum L. commonly known as February daphne or mezereon is a decorative shrub from the family Thymelaeaceae. It is a valuable nursery plant due to its high ornamental values, but its propagation by traditional methods is ineffective, therefore, little profitable. Micropropagation may be a good alternative way to produce this ornamental shrub. The experiment aimed to examine the effect of cytokinins (meta-Topolin, benzyladenine, and zeatin) and other growth regulators (gibberellic acid GA3 and auxin 1-naphthalene acetic acid NAA) added to the Murashige and Skoog (MS) and Woody Plant Medium (WPM) nutrients on regeneration of explants of D. mezereum ‘Alba’. In the all treatments containing plant growth regulators (PGR), 100% regeneration of explants has been observed. The highest number of shoots, after 6 weeks of culture, was produced on MS medium with 1 mg·L−1 BA and 0.1 mg·L−1 NAA. The longest shoots were produced on MS medium with 0.1 mg·L−1 of gibberellic acid (GA3) and 1 mg·L−1 of meta-Topolin (mT). Generally, the type and concentration of plant growth regulators had an essential effect on regeneration and growth of shoots of Daphne mezereum ‘Alba’ in the in vitro culture.
Highlights
February daphne Daphne mezereum L. belonging to the family Thymelaeaceae is legally protected in Poland
According to the visual observations, the Woody Plant Medium (WPM) composition is suitable for regeneration of D. mezereum ‘Alba’ during the long-term culture and only in combination with meta-Topolin
On WPM, the highest mT concentration resulted in a decreased shoot number relative to that obtained by the lower cytokinin concentrations
Summary
February daphne Daphne mezereum L. belonging to the family Thymelaeaceae is legally protected in Poland. It needs to be preserved as its habitat, i.e., deciduous forests are being replaced by conifers, the shrub flowering branches are torn away and plants are dug out of natural stands and transferred into gardens (Piekos-Mirkowa and Mirek 2006). Apart from flowers which appear very early in spring plants produce ornamental red berries of 1 cm in diameter which ripen at the end of June. Other propagation methods of this valuable species are being sought and one of the solutions may be tissue culture. According to Marks (1997), trials concentrate mainly on two species: D. × burkwoodii and D. odora, but due to the growing interest in other ornamental daphne species, the tissue According to Marks (1997), trials concentrate mainly on two species: D. × burkwoodii and D. odora, but due to the growing interest in other ornamental daphne species, the tissue
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