Abstract
To provide safer food, many technologies have been used to preserve food. One such technology is cold plasma, which can reduce viable bacterial counts in various food matrices. However, bacterial communities in food matrices before and after cold plasma treatment have not been investigated. In this communication, the EcoPlates™ were used to physiologically profile bacterial communities from poultry ground meat treated with rosemary, cold plasma or both. The cultures in the plates were incubated at 25 °C for seven days in an OmniLog® system. Responses of the bacterial communities to 31 chemicals were measured on formazan production. The results show that the three parameters of the Gompertz growth curves were observed in all samples, 2-hydroxybenzoic acid could not be used, while pyruvic acid methyl ester was used for a carbon source by the bacterial communities from all meat samples, each bacterial community metabolized different numbers of chemical compounds at different rates, and reduction of bacterial functional diversity was observed in the poultry meat samples treated with cold plasma and rosemary. In the future, investigations on whether the physiological profiling in bacterial communities be used as an indicator for effectiveness of cold plasma treatment of meat samples.
Highlights
Food safety is one of important health issues worldwide
Ground meat samples were divided into treatment groups as follows: (1) meat with no treatment as a control (C), (2) meat with cold plasma treatment (CP), (3) meat with rosemary treatment (R) and (4) meat with rosemary and cold plasma treatment (RP)
Further analysis on population sizes of bacterial communities of the samples after five-day storage at 4 C, there were statistically significant differences in the C5 vs. CP5 and C5 vs. RP5 groups, but not the C5 vs. R5 groups. These results of growth kinetics in the bacterial communities from the poultry ground meat samples with various treatments suggest that (1) as expected, bacteria in the meat samples were able to continue to grow during storage at 4 C, and (2) the Groups Rosemary1 Cold Plasma1 Storage (Day) 1 Growth Rate (Hour
Summary
In order to prevent food spoilage and preserve food quality and sanitary conditions for human consumption, many physical, chemical and biological treatments of food with various technologies have been explored [1, 2, 3] One such technology is plasma, the fourth fundamental state of matter, that consists of highly charged particles and unbound electrons [4, 5]. Based on the temperature of the medium, plasma can be classified into cold and hot plasmas [4, 6, 7] The latter means the temperature of the electrons, the ions and the neutrals are all the same, and the gas molecules in the hot plasma are fully ionized [4, 6]. Only a small fraction of the gas molecules is ionized [4, 6]
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