The effect of red Turi leaf extract (Sesbania glandiflora L.Pers) on the reduced levels of TNF-α, IL-1β and the number of bacterial colonies on the puerperium Mus Musculus ovarian inoculated by Staphylococcus aureus

Abstract

Staphylococcus aureus is one of the bacteria that causes postpartum infection, where this bacterium releases peptidoglycans and lipoteichoic acid (LTA) that start the inflammation response, which is then responded by macrophages by releasing pro-inflammation cytokines such as Tumor Necrosis Factor-Alpha (TNF-α) and Interleukin 1-Beta (IL-1β). Red Turi leaf extract, which is known to contain saponins, flavonoids, and tannins which work by suppressing the growth of bacteria and forcing bacterial cells to undergo lysis, causing the reduction of immune system activation which results in the reduction of secretion of pro-inflammation cytokines such as TNF-α and IL-1β. This research aims to analyze the effect of red Turi leaf extract in reducing TNF-α, IL-1β, and bacterial colonies on the puerperium Mus Musculus Ovarian Inoculated by Staphylococcus aureus. Results in this study proved that red Turi leaf extract of doses of 125, 250, and 500 mg/kgBW/day was able to reduce TNF-α, IL-1β, and the number of bacterial colonies on the puerperium Mus Musculus Ovarian Inoculated by Staphylococcus aureus. The optimum doses to reduce the number of bacterial colonies were doses of 250 and 500 mg/kgBW/day. Thus, the infection caused by intravaginal inoculation Staphylococcus aureus for 24 hours on the puerperium Mus Musculus Ovary was alleviated by treatment with red Turi leaf extract by reducing TNF-α, IL-1β, and the number of bacterial colonies on the puerperium Mus Musculus Ovarian Inoculated by Staphylococcus aureus.