Differences in the number of bacteria colony in saliva before and after pit and fissure sealant application

Abstract

The level of oral health is strongly influenced by the level of bacterial colonies in saliva. One of the diseases in the oral cavity that can occur as a result of bacterial growth is dental caries. One of the precautions to prevent caries is by using the application of pit and fissure sealant. The effective material as a pit and fissure sealant is Bis-GMA. The purpose of this study was to determine differences in the number of salivary bacterial colonies before and after application of pit and fissure sealant. The research was a quasi experimental study. The study design used is pre and post test with control group design using the research subject pediatric patients age 6-12 years old in one of the dental clinic in Makassar and the subject meet the criteria. The process of saliva gathering is done before and after the application of pit and fissure sealant from 15 pediatric patients which was bred to 30 salivary bacterial colonies in the laboratory of Microbiology, Faculty of Medicine, Hasanuddin University. Salivary bacterial colonies that have been bred consisted of 15 salivary bacterial colonies before treatment and 15 salivary bacterial colonies after treatment. Calculation of the number of salivary bacterial colonies is done by using the CFU / ml counting method. Statistical analysis was performed using the Wilcoxon signed-rank test in SPSS. The results of Wilcoxon signed-rank test to see the difference in the number of salivary bacterial colonies before and after application of pit and fissure sealant obtained a significant result (p<0.05). There is a significant difference in the number of bacterial colonies in saliva before and after application of pit and fissure sealant that is visible from the decrease in the number of bacterial colonies in saliva after the application of pit and fissure sealant.