The Effect of Rat Tympanic Membrane Perforation on Oxidants/Antioxidants Balance

Abstract

Myringosclerosis is a common sequel of the ventilation tube treatment of otitis media. Recent studies have established the relationship between reactive oxygen species (ROS) and myringosclerosis.The aim of this experimental study was to investigate the temporal change of oxidants/antioxidants balance at 6, 12, 24, and 48 hours after the myringotomy. Fifty healthy adult male Wistar albino rats, weighing approximate 250-300 g, were randomly separated into five groups (n = 10 rats/group): first group ,of control, nonmyringotimized and four groups myringotimized unilaterally on the left ear. The tympanic membranes were collected from sacrificed rats for biochemical evaluation of parameters of oxidants/antioxidants balance from 1st group and in 6 hours from 2nd group, in 12 hours from 3rd group, in 24 hours from 4th group and in 48 hours from 5th group. The index oxidants were malondialdehyde and carbonyl proteins and the index antioxidants were hydrogen donor ability and sulfhydril groups. Results and conclusions: The changes of oxidants/antioxidants balance and oxidative stress appear after myringotomy in 6 hours and increase in 12, 24, and 48 hours. Myringosclerosis develops promptly after myringotomy.