The effect of rat bone marrow-derived mesenchymal stem cells on proliferation and angiogenesis of endothelial cells

Abstract

Objective To investigate the effect of rat bone marrow-derived mesenchvmal stem cells (MSCs) on proliferation and angiogenesis of endothelial cells (ECs).Methods The rat bone marrow-derived mononuclear cells (BM-MNCs) were collected by density gradient centrifugation and cultured in different medium (DMEM and EGM-2) before identified by flow cytometry or immunofluorescenee.After direct and indirect co-culture,they were analysised by vascular network formation in vitro and immunofluorescence.Results Compared with DMEM,the BM-MNCs could formed typical colony-forming cells in EGM-2.After cuhured in DMEM and EGM-2 respectively,MNCs could develop into MSCs with greater than 95％ of cells expressing CD105,CD90 and CD73,and ECs with greater than 90％ of cells positive staining for CD31.Compared with indirect co-culture,direct co-culture of MSCs and ECs cells would result in significant reduction of tube quantity and tabe length (P ＜ 0.05).Furthermore,immunofluorescence staining demonstrated the number of ECs also significantly reduced (P ＜ 0.05).Conclusion Directly cocultured MSCs were able to inhibit ECs proliferation and vascular network formation. Key words: Rat bone marrow stem cells; Co-culture; Vascular network formation; Proliferation