The effect of progesterone injection combined with prostaglandineF2α, GnRH and hCG administrations on the pregnancy and lambing rate of lactating and non-lactating fat-tailed ewes during the breeding season.

Abstract

The objective was to compare the effect of human chorionic gonadotropin (hCG) and gonadotropin-releasing hormone (GnRH) on the pregnancy and lambing rate of treated ewes with a short-term progesterone (P4) injection during the breeding season. In Exp 1, non-lactating ewes (n=158) were used and received P4 , three times every 48h and received PGF2α along with the last dose of P4. Ewe received hCG (n=79, 3PHCG, IM) or GnRH (n=79, 3PGnRH, IM) 24 h after the last dose of P4 treatment. In the Exp 2, lactating ewes (n=62) received P4 and hCG (n=24, 3PHCG) or GnRH (n=24, 3PGnRH) similar to Exp 1, or considered as control (n=14) and received PGF2α 48 h before ram release. Rams were released into the studied ewe's flock 24 h after the hCG or GnRH administration, and pregnancy diagnosis was performed on day 50 after ram release. In Exp 1, all reproductive indices were not significant between groups except twin lambing rate, that was higher in the 3PHCG (11.3%) compared with the 3PGnRH (1.9%) group (p=.05). There were no significant differences in overall pregnancy and lambing rates between 3PHCG (70.9% and 67.1%) and 3PGnRH (69.6% and 68.4%) groups (p > .05). In Exp 2, all reproductive parameters were not significant between 3PHCG and 3PGnRH groups (p > .05). There was significant difference in overall oestrous rate between control (35.7%) and treatment (3PHCG, 70.9% and 3PGnRH, 79.2%) groups. To conclude, administration of GnRH can be a good alternative to hCG injection under field conditions.