Abstract
BackgroundExercise training is known as a practical way to increase cardioprotection against stress, and it seems that stem cell recruitment is one of its mechanisms. The purpose of the present study was to investigate the effect of preconditioning with High-intensity interval training (HIIT) on tissue levels of G-CSF, its receptor and C-Kit following acute myocardial infarction in male rats.MethodsTwenty Male Wistar rats were randomly divided into 4 groups of control, MI, HIIT, and HIIT+MI. Training groups performed 2 weeks of high intensity interval training in 4 sections. The first section consisted training in 3 days and 2 sessions in each day (4 × 2 min with 35–40 m/min and 3 × 2 min with 25–30 m/min between high intervals. The second part included 2 days of training (4 × 2 min with 40 to 45 m/min and 3 × 2 min with 28 to 32 m /min). The third part was performed in 3 days with one more repetition. The fourth section consisted 2 days of training and with one more repetition compared to section 3. For induction of myocardial infarction, subcutaneous injection of isoprenaline was used. CK, total CK, LDH, and troponin T were measured in serum and G-CSF, G-CSFR and C-Kit proteins were measured by the Western Blot method in the heart tissue.ResultsThe results of this study showed that enzymes of CK, total CK, LDH, troponin T had a significant increase in both MI and HIIT+MI groups compared to the other two groups (P < 0.001) and these indices in the MI group were significantly higher than the HIIT+MI group. Also, the results demonstrated that G-CSF, G-CSFR and C-Kit protein expression in the heart tissue significantly increased after MI. As well as, 2 weeks of HIIT training significantly increased G-CSF and C-kit in the training group compared to the control group, but the training caused that these proteins does not increase in HIIT+MI group as much as MI group.ConclusionsAlong with other protective pathways, high intensity interval training can increase cardioprotection and decrease heart injuries through the increase in G-CSF, G-CSFR and C-kit level.
Highlights
Exercise training is known as a practical way to increase cardioprotection against stress, and it seems that stem cell recruitment is one of its mechanisms
Serum markers of cellular injury and confirmation of necrosis and fibrous As shown in Fig. 2, the results of this study showed a significant increase in lactate dehydrogenase (LDH), CKMB, creatine kinase (CK) total and troponin-T following 2 weeks of High-intensity interval training (HIIT) and infarction (P < 0.001)
The results showed that the exercise training does not increase these cell injury indices in the HIIT+Myocardial infarcted (MI) group than in the MI group, which indicates the cardioprotection effect of the exercise training
Summary
Exercise training is known as a practical way to increase cardioprotection against stress, and it seems that stem cell recruitment is one of its mechanisms. The heart proper function depends on the delivery of blood and oxygen by the coronary arteries. Some factors, such as arteriosclerosis or the formation of clots in these vessels lead to an abnormal blood supply and ischemia of the heart muscle, resulting in substantial injury to this sensitive tissue. Recent studies have shown that increase of stem cell recruitment factors (such as Granulocyte colonystimulating factor (G-CSF), Stromal cell-derived factor (SDF), Stem cell factor (SCF), and FMS-like tyrosine kinase 3 (Flt3)), as well as increased stem cells, acts as a protective mechanism of the heart against infarction and improves the function of the left ventricle [5,6,7]. The results show that stem cells are as a novel method to treat heart disease [5,6,7]
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