The effect of poly (2-hydroxyethyl methacrylate) on the activity of matrix metalloproteinases in dentin

Abstract

Objective The purpose of this study was to test the effect of poly (2-hydroxyethyl methacrylate) on the matrix metalloproteinase in dentin. Methods PHEMA was prepared by the synthesis of methyl methacrylate by Bulk polymerization. Meanwhile, human dentin powder was prepared and divided into 6 groups as follows: The group without the treatment of PHEMA was used for control samples, and the other experimental groups were treated with different heights of PHEMA. Extracting the dentin protein from the six groups, the concentration of ICTP in the sample was analyzed by Cross-linked carboxyterminaltelopeptide typeⅠ collagen reagent box. The software of SPSS 17.0 was used for statistical analysis. ANOVA One-Way were used to analyze the effects of PHEMA and time on the ICTP, and LSD-t test for comparison between two groups. As well as the activity of MMP-2, 8, 9 in dentin was detection by Zymography, Using the gray method for image analysis through the J Image analysis software. Results There was a positive correlation between the concentration of ICTP and the activity of MMPs. PHEMA can inhibit the concentration of ICTP (F = 26.792, P = 0.001) and the effect of inhibition was decreased by increasing time. PHEMA can inhibit the activity of MMP-2 (F = 15.317, P = 0.0009) and MMP-8 (F = 6.475, P = 0.004) , the effect of inhibition was increased by increasing the contact area; the activity of MMP-9 was not significantly inhibited by PHEMA. Conclusion PHEMA can effectively inhibit the activity of MMP-2, 8 of the dentin, and the inhibition increased with the increase of contact area. Key words: Hydroxyethyl Methacrylates; Matrix metalloproteinases; Dentin; Cross-linked carboxyterminaltelopeptide of typeⅠ collagen