Abstract
Phenol was added in cultures of PK-15 cell line, at cell passage and on formed monolayer, in concentrations of 1/100, 1/500, 1/1000, 1/5000, 1/10000,1/15000 and 1/20000, in medium (g/ml). The cultures were incubated at 37,5°C, in 5% CO2, up to 24 hours. Untreated cultures served as control. With the decreasing concentration, the addition of Phenol at cell passage has totally or partially blocked the monolayer formation, about 90% formed monolayer being observed at concentration of 1/20000. Added on formed monolayer, Phenol has produced partial detachment of cells up to the concentration of 1/1000. At 1/5000, the monolayer remained relative normal. According to experimental results, Phenol elicits a strong cytotoxic effect on PK-15 cell culture
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Schedule a callMore From: Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca Veterinary Medicine
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