The effect of organic sources on the electron distribution and N2O emission in sulfur-driven autotrophic denitrification biofilters

Abstract

Sulfur-driven autotrophic denitrification (SAD) is considered as an effective alternative to traditional heterotrophic denitrification (HD) due to its cheap, low sludge production and non-toxicity. Nitrous oxide (N2O) as an intermediate product inevitably was generated at the limited supply of electron donor or unbalanced electron distribution condition during the denitrification process. Recently, autotrophic denitrification biofilters were conclusively implemented for advanced nitrogen removal in wastewater treatment plant (WWTP). However, residual organic sources after wastewater treatment could affect the electron distribution among denitrifying reductases and few studies are known about this issue. In this study, several lab-scale biofilters packed with elemental sulfur slices were applied to explore the electron distribution characteristics of autotrophic denitrification through the combination of different nitrogen oxides (NOx). The results clearly delineated that the different combination of nitrogen oxides had a remarkable effect on the electron distribution. In any case, the electrons likely flow toward nitrate reductase (Nar) under a single nitrogen oxide combination, followed by nitrite reductase (Nir) and nitrous oxide reductase (Nos). The concurrent presence of multiple electron acceptors resulted in most electrons flowing toward Nar, and least toward Nos. Compared to traditional SAD, the reduction rate of nitrogen oxide in the sulfur-driven autotrophic denitrification with influent of organic source (OSAD) was greatly improved. The maximum value of the true specific rates of NO3− in OSAD process was 9.43 mg-N/g-VSS/h. It was increased by 8.26 folds higher than that in traditional SAD. The electrons were more easily distributed to Nos with the addition of sodium acetate, which further promoted the N2O reduction. This study will provide theoretical support for controlling N2O release in SAD biofilters.