The effect of octamer-binding transcription factor 4 pseudogene 5 on bladder cancer cell line T24 migration and invasion

Abstract

Objective To investigate octamer-binding transcription factor 4 pseudogene 5 (OCT4-pg5) expression in bladder cancer cell lines and its effect on T24 migration and invasion and the potential mechanism. Methods The real-time quantitative reverse transeriptase-polymerase chain reaction (RT-qPCR) was conducted to detect OCT4-pg5 expression in 5 kinds of bladder cancer cell lines (5637, EJ, BIU-87, T24, TCCSUP) and one normal Bladder epithelial cell line SV-HUC-1. Si-OCT4-pg5 vector and its control vector were transfected into T24 cells by Lipofectamine 2000, and OCT4-pg5 expression was detected by RT-qPCR. Next, wound healing assay and Transwell assay were used to examine the effects of OCT4-pg5 on T24 cell migration and invasion. Moreover, the possible mechanism by which OCT4-pg5 mediated T24 migration and invasion was explored using Western blotting and RT-qPCR. Results In comparison to SV-HUC-1, OCT4-pg5 levels were up-regulated in bladder cancer cell lines, especially in T24 cell (P<0.01). RT-qPCR results showed that OCT4-pg5 espression was obviously down-regulated after being translated with si-OCT4-pg5 vector. OCT4-pg5 down-expression in T24 cell could inhibit cell migration[ (28.50±4.08)% and (43.26±5.49)%, P<0.01] and invasion [ (104.50±6.95) and (140.50±8.90) cells, P<0.01]. In addition, down-regulation of OCT4-pg5 significantly reduced Vimentin (0.60±0.06) and β-catenin (0.70±0.08) mRNA expression, and improved E-cadherin mRNA expression (1.50±0.10) when compared with their contral groups (1.00±0.02, 1.00±0.04 and 1.00±0.05, respectively, P<0.01). Moreover, the same results were also obtained by western blotting. Conclusion OCT4-pg5 is overexpression in bladder cancer cells. And OCT4-pg5 down-expression in T24 cell could inhibit cell migration and invasion. OCT4-pg5 mediated T24 migration and invasion probably induced by regulating E-cadherin, Vimentin, β-catenin expression. Key words: Bladder cancer; Octamer-binding transcription factor 4 pseudogene 5; Migration; Invasion