Mechanism of long non-coding RNA SBF2-AS1 regulated AKT/FOXM1 signaling pathway on proliferation and apoptosis of bladder cancer cells by targeting microRNA-582-5p

Abstract

Objective To investigate the effects of long non-coding RNA (lncRNA) SBF2-AS1 on proliferation, migration and invasion of bladder cancer cells and its potential molecular mechanism. Methods MTT assay was used to determine the proliferation activity of bladder cancer cell line. Transwell assay was used to determine cell migration and invasion. Dual-luciferase reporter assay system was used to verify the regulatory relationship between SBF2-AS1 and microRNA-582-5p (miR-582-5p) . qRT-PCR was used to examine the RNA expression of SBF2-AS1 and miR-582-5p. Results Compared with normal bladder epithelial cells, the expression level of SBF2-AS1 in bladder cancer cell lines T24, SW780 and 5637 significantly increased (P<0.05) , whereas the expression level of miR-582-5p significantly decreased (P<0.05) . Inhibition of SBF2-AS1 expression inhibited T24 cell proliferation, migration and invasion. Dual-luciferase reporter assay system showed that SBF2-AS1 targeted negative regulation of miR-582-5p expression. Overexpression of miR-582-5p inhibited proliferation, migration and invasion of bladder cancer cell line T24. Inhibition of miR-582-5p expression reversed the inhibitory effect of silenced SBF2-AS1 expression on proliferation, migration and invasion of bladder cancer cell line T24. Conclusion SBF2-AS1 inhibits the proliferation, migration and invasion of bladder cancer cell line T24 by up-regulating miR-582-5p expression. Key words: Bladder cancer; SBF2-AS1; miR-582-5p; Proliferation; Migration; Invasion