Abstract
in this work were studied the characteristics of the development of F. tataricum and F. esculentum callus cultures in vitro when they were treated with chemical mutagen solutions - MH, MNU (0.05; 0.1; 0.2; 0.5; 1; 2 mM). When compared to the control on RX medium, the calli treated with the MNU mutagen died more often at higher concentrations (0.5; 1; 2 mM), indicating the effectiveness of the mutagenic effect. The survival rate of callus cultures subcultivated on a medium containing lower concentrations of mutagens (0.05; 0.1; 0.2 mM) was quite high when compared to higher concentrations, but low when compared to the control. The role of mutagenic factors in increasing genotypic variability, as well as the method of obtaining a new source material for various aspects of the buckwheat breeding process, have not been studied.
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