Abstract
Eryngium planum L. cell and organ cultures were maintained on Murashige and Skoog media (MS), supplemented with exogenous hormones of different types and various concentrations for high biomass growth. The callus and cell suspension cultures were treated with increased sucrose concentration and/or elicited by methyl jasmonate for the enhancement of selected phenolic acids accumulation. Three phenolic acids, rosmarinic acid (RA), chlorogenic acid (CGA) and caffeic acid (CA), were detected by HPLC-DAD in those cultures. The sum of their content in the dry material was found to be higher in the shoot culture (3.95 mg g−1), root culture (7.05 mg g−1), callus (6.20 mg g−1) and cell suspension (2.04 mg g−1) than in the leaves (1.87 mg g−1) and roots (0.76 mg g−1) of intact plants. The major compound of in vitro cultures was always rosmarinic acid. The content of RA could be increased approximately threefold (16.24 mg g−1) in the callus culture and approximately twofold (3.91 mg g−1) in the cell suspension culture by elicitation with 100 μM methyl jasmonate (MeJA). The higher concentration of sucrose (S) in the medium (5, 6 %) led to over a twofold increase of CGA content in the callus culture (2.54 mg g−1). The three mentioned phenolic acids have been found in E. planum undifferentiated and differentiated in vitro cultures for the first time.
Highlights
Eryngium planum L. (Flat Sea Holly), a species that belongs to the Apiaceae family and to the Saniculoideae subfamily, has been reported as a medicinal plant used in traditional medicine in Europe (E. plani herba and E. plani radix)
Cell suspension and organ cultures were established to study the accumulation of chosen phenolic acids
The elicitation with 100 lM methyl jasmonate (MeJA) of callus growing on MS medium supplemented with 4 % S enhanced 2.5-fold total phenolic acid production and threefold of rosmarinic acid (RA) accumulation compared to the control e.g. callus on MS with 3 % S
Summary
Eryngium planum L. (Flat Sea Holly), a species that belongs to the Apiaceae family and to the Saniculoideae subfamily, has been reported as a medicinal plant used in traditional medicine in Europe (E. plani herba and E. plani radix). The callus and cell suspension cultures were treated with increased sucrose concentration and/or elicited by methyl jasmonate for the enhancement of selected phenolic acids accumulation. The aim of the present experiment was to establish the undifferentiated (callus and cell suspension) and organ (shoot and root) cultures of E. planum and to test their ability to synthesise phenolic acids RA, CGA and CA.
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