Abstract
Objective: Lycopene is a carotenoid with anti-inflammatory and antioxidant properties. The aim of this study was to determine the effects of lycopene on oxidative DNA damage levels in experimental diabetic rats. Subjects and Methods: Four experimental groups, each consisting of 7 rats, were prepared as Controls, Diabetes (D), Lycopene-treated diabetes (DL) and Lycopene (L). STZ (45 mg/kg) was administered to the diabetic groups intraperitoneally in a single dose. Lycopene was administered to the L and DL groups (10 mg lycopene/kg/day). The test procedure continued for four weeks. To understand the occurrence of diabetic conditions, serum glucose and HbA1c% in the whole blood were determined. The 8-OHdG levels, a marker of oxidative DNA damage, were determined in the blood serum. Results: Blood glucose and HbA1c% were higher in the DL group than in the control group and L group (p <0.05) and lower in the D group (p <0.05). 8-OHdG levels were higher in D group than the other groups (p <0.05) while 8-OHdG levels in DL group were lower than D group (p <0.05) and approximated to the control group. Conclusion: It can be suggested that lycopene may be described as a protective agent to prevent oxidative DNA damage originated from diabetes.
Highlights
IntroductionAll the changes in the molecular integrity of the genetic material with the effect of exogenous or endogenous factors are referred as “DNA damage”
Diabetes mellitus is a widespread disease with high morbidity and early mortality rates leading to vascular, renal, retinal or neuropathic disorders and acute metabolic complications due to prolonged hyperglycemia [1].All the changes in the molecular integrity of the genetic material with the effect of exogenous or endogenous factors are referred as “DNA damage”
Oxidative stress plays an important role in Type 2 diabetes and pathogenesis of its complications
Summary
All the changes in the molecular integrity of the genetic material with the effect of exogenous or endogenous factors are referred as “DNA damage”. Reactive oxygen species such as superoxide and hydroxyl radicals create various lesions which include 8-oxoguanine. It is known that oxidative stress induces oxidative DNA damage, which may lead to mutagenesis [2 - 4]. 8-OHdG is the most commonly known oxidative DNA damage biomarker formed through free radicals and the. Oxidative stress plays an important role in Type 2 diabetes and pathogenesis of its complications. The studies conducted for this purpose reported that 8-OHdG is significant for the measurement of biomarkers such as malondialdehyde (MDA) and determining the status of oxidative stress in organisms [9]
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