Abstract
Aim of this study was to investigate the effects of lipoic acid on uterine wound healing by immunohistochemical and biochemical assay in a rat uterine horn model with full thickness injury. Thirty-two female Wistar albino rats were randomised into five groups: Control group, with no intervention; uterine scar group 15days (US15d), uterine scar group 15days+alpha lipoic acid (ALA) (US15d+ALA), uterine scar group 30days (US30d) and uterine scar group 30days+ALA (US30days+ALA). After uterine incision 100mg/kg of ALA was administered by oral gavage for either 15 or 30days. Vascular endothelial growth factor (VEGF) and alpha smooth muscle actin (α-SMA) distribution were evaluated by immunohistochemical methods in tissue and ELISA methods in tissue homogenate. The percentage of α-SMA positive area in US15d+ALA and US30d+ALA groups was significantly higher than US15 and US30d groups. The percentage of VEGF positive area in US15d+ALA group was significantly higher than US15d group and US30d+ALA group was significantly higher than US30d group. Biochemically, α-SMA was significantly higher in the US15d+ALA group when compared to US15d group and higher in US30d+ALA group when compared to US30d group. VEGF was significantly higher in US15d+ALA and US30d+ALA groups when compared to US15 and US30d groups. In conclusion, ALA was found to be effective in enhancing wound healing in uterine full thickness injury.
Published Version
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