The Effect of Lamium album Extract on Cultivated Human Corneal Epithelial Cells (10.014 pRSV-T).

Roman Paduch,Anna Woźniak

doi:10.4103/2008-322x.170349

Abstract

Purpose:To evaluate the effect of Lamium album extract on human corneal epithelial cells (10.014 pRSV-T cell line) cultured in vitro.Methods:Normal human corneal epithelial cells were incubated with ethanol, ethyl acetate and heptane extracts from Lamium album. Their effect on cells was evaluated by neutral red (NR) uptake and MTT assays for cytotoxicity, ELISA for immunomodulation, Griess method for nitric oxide levels, DPPH assay for free radicals scavenging activity. A blank control consisted only of culture medium.Results:In NR and MTT assays, Lamium album extracts did not affect cell viability (80% at 125 μg/ml concentration). Ethanol was the least toxic extract (cell viability over 88%) and expressed the most potent reactive oxygen species (ROS) scavenging action. It was 19.88 ± 0.87% higher than controls representing a reduction corresponding to 7.136 μg/ml of trolox. Heptane extract revealed no ROS scavenging activity. All extracts decreased NO production by cells. The most active extract was ethanol (8 μg/ml) which reduced NO level to 0.242 μM (75% decrease compared to control). Extracts influenced pro-inflammatory (IL-1, IL-6, TNF-α) and anti-inflammatory (IL-10) cytokines levels reducing all of them in general. The strongest reduction in tested cytokines level was observed by the heptane extract. On the other hand, the ethanol extract induced mainly TNF-α level in a concentration dependent manner.Conclusion:Selected Lamium album extracts influence human corneal epithelial cells. Generally, while not toxic, they modulate pro-inflammatory and anti-inflammatory cytokines levels, and decrease NO release by cells; moreover, ethanol and ethyl acetate extracts reduce ROS levels.

Highlights

The human cornea, the outer transparent part of the eye, is constantly exposed to the external environment,[1,2] and as a highly specialized organ requires specific conditions to function properly and provide ideal vision.[3]
Viability of corneal epithelial cells did not decrease below 88% at the applied concentrations
The beneficial effects of Lamium album extracts were evaluated as a possible natural protective agent for human corneal epithelial cells

Summary

INTRODUCTION

The human cornea, the outer transparent part of the eye, is constantly exposed to the external environment,[1,2] and as a highly specialized organ requires specific conditions to function properly and provide ideal vision.[3] Continuous exposure of the corneal epithelium. Effect of Lamium album Extract on Cultivated Corneal Epithelial Cells; Paduch and Woźniak to stressors including mechanical injury, chemical and biological toxins, bacterial and fungal infections, and radiations such as ultraviolet light, may adversely affect eye function and even lead to loss of vision.[2] any inflammation and damage to the corneal epithelial lining should be quickly but gently eliminated to maintain corneal transparency. The present study was conducted to evaluate the cytotoxic, anti‐inflammatory and anti‐oxidant effects of ethanol, ethyl acetate and heptane extracts of Lamium album on cultivated human corneal epithelial cells (10.014 pRSV‐T cell line)

METHODS

RESULTS

IL - 10

DISCUSSION