Abstract
Background: Dental caries and decay caused by Streptococcus mutans. Sucrose, stimulates S. mutans to yield insoluble glucans to form oral biofilm also called dental plaque to start process of caries. The GtfB and LuxS genes of S. mutans are responsible for development and evolution of biofilm. Lactobacillus plantarum as probiotic can affect the biofilm formation of S. mutans.Aim of the study: To evaluate the effect of Lactobacillus plantarum against S. mutans biofilm formation.Results: Growing biofilm in the presence of sucrose was detected using 100 well microtiter plate crystal violet assay and biofilm formation by S. mutans in the presence of Lactobacillus was detected. Gene expression of biofilm forming genes (GtfB and LuxS) was quantified through Real- time PCR. The transcriptional levels of GtfB and LuxS genes were remarkably down regulated 9.43 -and 4.24- fold change, respectively, when treated with lactobacillus plantarum in comparison with the control group.Conclusions: Lactobacillus plantarum as probiotics can be used effectively to reduce the chance of dental caries by decreasing the effects of S. mutans and their gene expression to conserve good oral health
Highlights
T ooth decay, caries and periodontal disease are very common health problem that cost a lot in the world. [1]Developed countries have addressed this health problem and controlled it with fluoride enriched water and personal hygiene products since early in the 1960s.[1]
For Biofilm assays S.mutans was grown in Brain Heart Infusion (BHI) containing 2% sucrose while Lactobacillus were grown in MRS broth with or without sucrose
Biofilm Formation: The results of biofilm formation of S. mutans indicates that most strains (80%) produce strong biofilm (Table-2)
Summary
Developed countries have addressed this health problem and controlled it with fluoride enriched water and personal hygiene products since early in the 1960s.[1] Dental disease remains a “soundless epidemic” in the world that threatens children and adults.[1,2] The oral streptococci principally mutans streptococci are related with the development of caries in humans.[3] The adhesion of oral streptococci such as Streptococcus mutans to tooth surfaces has the chief role in their pathogenicity. [13] the regulatory effect of lactobacillus plantarum on S. mutans biofilm formation that is closely associated with dental caries is rarely studied. In this study we tried to evaluate the effects of Lactobacillus plantarum on S. mutans dental biofilm formation. Aim of the study: To evaluate the effect of Lactobacillus plantarum against S. mutans biofilm formation. Conclusions: Lactobacillus plantarum as probiotics can be used effectively to reduce the chance of dental caries by decreasing the effects of S. mutans and their gene expression to conserve good oral health.
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