Abstract
The study aimed to know the effect of Kebar grass extraction amounts of mice (Mus musculus) follicles ovary exposed by carbofuran. This study was an experimental study with a completely randomized design. Forty two lactation mice used in this study were divided into seven groups, that were: C was control (Aquadest), P1 was injected with carbofuran 1/4 LD50 (0.0125mg/day), P2 was injected with carbofuran 1/8 LD50 (0.00625mg/day), P3 was injected with carbofuran 1/4 LD50 (0.0125mg/day) and Kebar grass extract (0.135mg/day), P4 was injected with carbofuran 1/8 LD50 (0.00625mg/day) and Kebar grass extract (0.135mg/day), P5 was injected with carbofuran 1/4 LD50 (0.0125mg/day) and vitamin C (0.2ml/day) and P6 was injected with carbofuran 1/8 LD50 (0.00625mg/day) and vitamin C (0.2ml/day) with a treatment period of 14 days. Then on the 15day of treatment, mice were sacrificed, and the ovary was taken. Then, histology preparation with HE (Hematoxylin-eosin) staining was made, and follicles ovary were counted. Data were analyzed by One Way ANOVA followed by the Duncan test. This study showed that exposed carbofuran was a significant decrease in the growth of ovarian follicles (p<0.05). The administration of the Kebar grass extraction exposure to carbofuran is proven to increase primary and secondary follicles. The Kebar grass extraction administration gave a better effect than vitamin C (p<0.05).
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