Abstract
ABSTRACT Pigment mutant C-2A' from Scenedesmus obliquus which greens only in the light was submitted to intermittent light conditions (2 min white light (60 W/m2)/60 min darkness). Under these conditions only 10% of the total chlorophyll was formed compared to cells under continuous light, whereas chlorophyll b remained at the initial trace level. All chlorophyll-protein complexes of the light-harvesting complex were missing, while those related to PSII reaction centers increased. The capacities of PSI and PSII per chlorophyll were higher in cells partially greened under intermittent light than in those under continuous light. Transformation of cells from intermittent to continuous light caused a drastic increase in chlorophylls a and b above the normal level, establishing a chlorophyll a/b ratio and the light-harvesting complex pattern found in wild-type cells.
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