Abstract
1. 1. The effect of insulin on the incorporation of 32P i into the phospholipids of rat fat cells incubated in glucose-free medium has been investigated. 2. 2. The incorporation of 32P i into fat cell phospholipid increased with time, and incubation of fat cells with insulin resulted in increased incorporation of 32P i into phospholipid compared with controls. 3. 3. Fat cells incubated with insulin for I h showed increased specific radioactivities of phosphatidylcholine, phosphatidylethanolamine, phosphatidic acid, phosphatidylinositol and cardiolipin compared with controls. 4. 4. ATP concentrations in fat cells were determined by the luciferase assay. Insulin incubated for 1 h with fat cells produced no significant change in ATP concentrations compared with controls. Glucose incubated for I h with fat cells either with insulin or without insulin did not result in any change in ATP concentration compared with incubated controls. 5. 5. The effect of insulin on the incorporation of 32P i into ATP was determined and the specific radioactivity of ATP calculated. Incubation of fat cells with insulin resulted in an increase in the specific radioactivity of ATP compared with ATP in control cells, and the increase was time-dependent.
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